Ues, incHas an opportunity available for a research scientist, focused in biosignatures, to join our team working at wright patterson air force base in dayton, ohThis position will support development of ocular complex 3d models as a representative of ocular surface and tear secretory systemsResearch functions will include: design, construct, in vitro 3d corneal, lacrimal or conjunctiva equivalents, or organoids models that are innovative and advanced, promising to expand mechanistic studies to identify biosignatures of stress in tearsDesign, construct, in vitro three-dimensional (3d) coculture model for the ocular surface of tear secretionIt is composed of mammalian conjunctival epithelium and lacrimal gland cells that recapitulates the aqueous and mucin layers of the tear filmDesign and optimize a culture system for both cell types to optimize their secretory functions applying techniques involve goblet cell enrichment, air-lifting culture, and 3d spheroid formation techniques etcThe co-culture model of the two cell components produce tear secretion and expression of biosignatures as secretory stress markersExpose this model to various operationally relevant stress in laboratory conditions (extreme temperatures, hypoxia, particles, and chemicals) and measure relevant biosignaturesEssential functions for this role will include:
Design of in vitro 3d corneal, lacrimal or conjunctiva equivalents or organoids models including primary cell isolation from tissues and cells culturing
Perform imaging techniques (confocal, optical and electron microscopy), molecular tools (pcr, elisa etc.) and physiology tools (ocular health, mechanism of secretion) to evaluate 3d models in various operationally relevant stress conditions
Utilize crispr/cas9 gene editing tools, mirna isolation, gene expression, protein analysis, exosomes/microvesicle characterization to validate models
Data analysis, results interpretation, statistical analysis, and scientific communication
Project development, troubleshooting, presenting
Requirements:
Ph.dIn related field (biochemistry, molecular biology, biomedical engineering, cell biology) is required
1-5 years of relevant research experience.
Proficiency in ophthalmology, imaging techniques and molecular skills are required.
A successful candidate should demonstrate well-developed planning and organizational skills with ability to prioritize multiple tasks and set/meet deadlines
A successful candidate will have excellent written and verbal communication skills with proven ability to produce clear, succinct reports.
Additionally, this role will provide laboratory and technical support to other staff
Additional information
Ues, incIs an innovative science and technology company providing customers with superior research and development expertise since its inception in 1973Our long-term success is a direct result of a strong commitment to the success of our employeesWe look forward to reviewing your application.
Ues, incIs committed to hiring and retaining a diverse workforceWe are proud to be an equal opportunity/affirmative action employer, making decisions without regard to race, color, religion, creed, sex, sexual orientation, gender identity, marital status, national origin, age, veteran status, disability, or any other protected classU.sCitizenship is required for most positions.
